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Product Details
Synonyms
HLA-A*0201 & B2M
Source
Unconjugated Human HLA-A*02:01&B2M Monomer Protein (HLM-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M)).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated. The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 38-42 kDa and 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Unconjugated Human HLA-A*02:01&B2M Monomer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Unconjugated Human HLA-A*02:01&B2M Monomer Protein (Cat. No. HLM-H52H5) is more than 85% and the molecular weight of this protein is around 48-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Unconjugated Human HLA-A*02:01&B2M Monomer Protein (Cat. No. HLM-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.5-4 ng/mL (QC tested).
Protocol
Immobilized Unconjugated Human HLA-A*02:01&B2M Monomer Protein (Cat. No. HLM-H52H5) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
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