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Your Position: Home > Protein > CD3E & CD3D > CDD-S52W3

Sus scrofa CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)

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  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon
  • Source
    Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free(CDD-S52W3) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Leu 116 (CD3E) & Tyr 22 - Leu 104 (CD3D) (Accession # NP_999392.1 (CD3E) & NP_998940.1 (CD3D)).
    Predicted N-terminus: Glu 23 (CD3E) & Tyr 22 (CD3D)
  • Molecular Characterization
    CD3E & CD3D Structure

    Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.2 kDa (CD3E) and 13.5 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

CD3E & CD3D MALS images

The purity of Sus scrofa CD3E&CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-S52W3) is more than 90% and the molecular weight of this protein is around 27-37 kDa verified by SEC-MALS.

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates

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Price(USD) : $368.00

Price(USD) : $3360.00

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