Sus scrofa CD3E&CD3G Heterodimer Protein, His Tag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 16.2 kDa (CD3E) and 13.5 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Sus scrofa CD3E&CD3G Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Sus scrofa CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (Cat. No. CDG-S52W3) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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