This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.5 kDa. The protein migrates as 47-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human SIRP alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human SIRP alpha, His Tag(Cat. No. SIA-H5225) was more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Loaded Human CD47, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H52A5) on Protein A Biosensor, can bind Human SIRP alpha, His Tag (Cat. No. SIA-H5225) with an affinity constant of 1.1 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Immobilized Human CD47, Fc Tag (Cat. No. CD7-H5256) on AHC Biosensor, can bind Human SIRP alpha, His Tag (Cat. No. SIA-H5225) with an affinity constant of 0.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS assay shows that Human SIRP alpha, His Tag (Cat. No. SIA-H5225) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL (Routinely tested).
FACS analysis shows that the binding of Human SIRP alpha, His Tag (Cat. No. SIA-H5225) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti-CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.2257 μg/mL (Routinely tested).
Authors: Yu XY, et al.
Journal: Biochimie 2018
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Price(USD) : $280.00
Price(USD) : $1980.00
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