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Your Position: Home > Protein > HLA-A*0201 | B2M | MAGE-A4 > HLM-H52H6

Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Monomer, MALS verified)

  • Source
    Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein(HLM-H52H6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & GVYDGREHTV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & GVYDGREHTV).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated.

    The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-45 kDa and 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
HLA-A*0201 | B2M | MAGE-A4 SDS-PAGE

Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
HLA-A*0201 | B2M | MAGE-A4 MALS images

The purity of Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H52H6) is more than 85% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0201 | B2M | MAGE-A4 ELISA

Immobilized Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H52H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    The MAGE A4 antigen is a cancer-testis antigen and is expressed intracellularly in various solid tumor tissues, MAGE A4230-239 peptide (GVYDGREHTV) is a cytotoxic T lymphocyte (CTL) epitope presented by HLA-A2 The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GVYDGREHTV peptide of the MAGE-A4.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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