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Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated.
The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-45 kDa and 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H52H6) is more than 85% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
Immobilized Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H52H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H52H6) at 1 μg/mL (100 μL/well) can bind Anti-HLA-A2&MAGE-A4 Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (QC tested).
Immobilized Unconjugated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Complex Protein (Cat. No. HLM-H52H6) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (Routinely tested).
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