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| Assay Type | Sandwich-ELISA |
| Analyte | HGF |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | 40 pg/mL |
| Standard Curve Range | 62.5 pg/mL-8000 pg/mL |
| Assay Time | 1 hr 45 min |
| Suitable Sample Type | For the quantitative determination of human HGF in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| ID | Components | Size |
| CRB012-C01 | Pre-coated Anti-HGF Antibody Microplate | 1 plate |
| CRB012-C02 | Human HGF Standard | 20 μg×2 |
| CRB012-C03 | Biotin-Anti-HGF Antibody Con. Solution | 100 μL |
| CRB012-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB012-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CRB012-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB012-C07 | 20× Washing Buffer | 50 mL |
| CRB012-C08 | Sample Dilution Buffer | 15 mL×2 |
| CRB012-C09 | Substrate Solution | 12 mL |
| CRB012-C10 | Stop Solution | 6 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human HGF in serum, plasma and cell culture supernates.
It is for research use only.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.
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