Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > HGF > CRS-B012

ClinMax™ Human HGF ELISA Kit

For research use only.

Order Now

Product Details
Assay TypeSandwich-ELISA
Sensitivity40 pg/mL
Standard Curve Range62.5 pg/mL-8000 pg/mL
Assay Time1 hr 45 min
Suitable Sample TypeFor the quantitative determination of human HGF in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Materials Provided
CRB012-C01Pre-coated Anti-HGF Antibody Microplate1 plate
CRB012-C02Human HGF Standard20 μg×2
CRB012-C03Biotin-Anti-HGF Antibody Con. Solution100 μL
CRB012-C04Biotin-Antibody Dilution Buffer8 mL
CRB012-C05Streptavidin-HRP Con. Solution500 μL
CRB012-C06Streptavidin-HRP Dilution Buffer15 mL
CRB012-C0720× Washing Buffer50 mL
CRB012-C08Sample Dilution Buffer15 mL×2
CRB012-C09Substrate Solution12 mL
CRB012-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human HGF that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human HGF in serum, plasma and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2℃-8℃ upon receiving. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
    This assay kit is used to measure the concentrations of human HGF by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- HGF Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-HGF Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Typical Data Please refer to DS document for the assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.

  • Background: HGF
    Hepatocyte growth factor, HGF
    Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.
  • Clinical and Translational Updates

Comments (0)

ETA of in-stock products: 2 business days

Price(USD) : $498.00

Price(USD) :

Promotion & Exhibitions

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message