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Your Position: Home > Kits > HGF > CRS-B012

ClinMax™ Human HGF ELISA Kit

For research use only.

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Product Details
Assay TypeSandwich-ELISA
Regulatory StatusRUO
Sensitivity40 pg/mL
Standard Curve Range62.5 pg/mL-8000 pg/mL
Assay Time1 hr 45 min
Suitable Sample TypeFor the quantitative determination of human HGF in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 uL
Materials Provided
CRB012-C01Pre-coated Anti-HGF Antibody Microplate1 plate
CRB012-C02Human HGF Standard20 μg×2
CRB012-C03Biotin-Anti-HGF Antibody Con. Solution100 μL
CRB012-C04Biotin-Antibody Dilution Buffer8 mL
CRB012-C05Streptavidin-HRP Con. Solution500 μL
CRB012-C06Streptavidin-HRP Dilution Buffer15 mL
CRB012-C0720× Washing Buffer50 mL
CRB012-C08Sample Dilution Buffer15 mL×2
CRB012-C09Substrate Solution12 mL
CRB012-C10Stop Solution6 mL
  • Background
    Human HGF ELISA Kit is a one-step quick immunoassay designed to measure human HGF levels in cell culture supernates, serum, and plasma. This assay kit is a standard sandwich-ELISA format. It contains recombinant human HGF and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human HGF concentrations. The specificity has been verified.
  • Application

    The kit is developed for quantitative detection of human HGF in serum, plasma and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Typical Data Please refer to DS document for The assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.

  • Background: HGF
    Hepatocyte growth factor, HGF
    Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.
  • Clinical and Translational Updates
  • Please contact us via if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $498.00

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