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Product Details
Synonyms
IgE
Source
Mouse IgE Fc Protein, His Tag (IGE-M52H3) is expressed from human 293 cells (HEK293). It contains AA Val 91 - Ser 421 (Accession # P06336).
Predicted N-terminus: Val 91
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.3 kDa. The protein migrates as 45-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse IgE Fc Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3) is more than 95% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3) at 5 μg/mL (100 μL/well) can bind Mouse Fc epsilon RI alpha,C-Fc with a linear range of 0.02-5 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human Fc epsilon RI alpha, His Tag (Cat. No. FCA-H5228) immobilized on CM5 Chip can bind Mouse IgE Fc Protein, His Tag (Cat. No. IGE-M52H3) with an affinity constant of 0.391 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Immunoglobulin E
- Category：
- Approved Drugs：
5 Details
- Drugs in Clinical Trials：
23 Details
- Highest Development Stage：
Approved
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