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Your Position: Home > Antibody > Adalimumab > ADB-BY17c

Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (recommended for PK/PD)

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  • Source
    Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (ADB-BY17c) is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293). The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Adalimumab ADA.
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Recognizes Adalimumab specifically, no cross reactivity with other humanized antibodies.
  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (13.5 angstroms) is used in this product to minimize potential steric hindrance.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
SDS-PAGE
Adalimumab SDS-PAGE

Biotinylated Anti-Adalimumab Antibody (22E7A4A2) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Adalimumab MALS images

The purity of Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Bioactivity-Elisa
 Adalimumab ELISA

Immobilized Adalimumab at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-Adalimumab Antibody (22E7A4A2) (Cat. No. ADB-BY17c) with a linear range of 0.4-3 ng/mL (QC tested).

  • Background
    Adalimumab is a human monoclonal antibody against TNF-alpha. It is produced by recombinant D technology using a mammalian cell expression system. It consists of 1330 amino acids and has a molecular weight of approximately 148 kilodaltons.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $1380.00

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