Biotinylated Human TROP-2 / TACSTD2 Protein, Fc,Avitag™

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Cat. No. / Size
Price
Qty
TR2-H82F4-25ug
$380.00
TR2-H82F4-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    TACSTD2, GA733-1, M1S1, TROP2

  • Source

    Biotinylated Human TROP-2 Protein, Fc,Avitag (TR2-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # P09758-1).

    Predicted N-terminus: Gln 31

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  • Molecular Characterization

    TROP-2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 55.6 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    TROP-2 SDS-PAGE

    Biotinylated Human TROP-2 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     TROP-2 ELISA

    Immobilized Mouse Monoclonal Antibody Against Human TROP-2 Protein,Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human TROP-2 Protein, Fc,Avitag (Cat. No. TR2-H82F4) with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol
  •  TROP-2 ELISA

    Immobilized Biotinylated Human TROP-2 Protein, Fc,Avitag (Cat. No. TR2-H82F4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Monoclonal Antibody Against Human TROP-2 Protein,Mouse IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

    Protocol

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Background

TROP-2 is a single-copy gene in human cells, and encodes a type-1 transmembrane glycoprotein which is over-expressed in various malignancies, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1. TROP-2 is related to epithelial cell adhesion molecule (EpCAM), also called TROP-1, gp40, and KSA. Trop-1 and Trop-2 are homologous to serum IGF-II-binding proteins and appear as signal transducers. Thus, they likely represent novel cell-surface receptors and may play a role in regulating the growth of carcinoma cells.

Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor-associated calcium signal transducer 2

  • Category:

  • Approved Drugs:

    3 Details

  • Drugs in Clinical Trials:

    47 Details

  • Highest Development Stage:

    Approved

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