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Your Position: Home > Antibody > IgG control > DNP-M553

Cynomolgus IgG1 Kappa Isotype Control (mAb)

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  • Source
    Cynomolgus IgG1 Kappa Isotype Control (mAb) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
  • Species

    Mouse

  • Isotype

    Cynomolgus IgG1 | Cynomolgus Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Cynomolgus

  • Specificity

    This product is a specific antibody specifically reacts with DNP.

  • Application
    ApplicationRecommended Usage
    ELISA1-5000 ng/mL
  • Purity

    >90% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IgG control SDS-PAGE

Cynomolgus IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 IgG control ELISA

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Cynomolgus IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M553) with a linear range of 0.001-0.25 μg/mL (QC tested).

  • Background
    A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
  • Clinical and Translational Updates

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