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Your Position: Home > Protein > Sonic Hedgehog > SH7-H5229

Human Sonic Hedgehog / Shh Protein, His Tag, premium grade

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  • Synonym
    SHH,HHG-1,Sonic hedgehog,HLP3,HPE3,SMMCI,TPT,TPTPS
  • Source
    Human Sonic Hedgehog, His Tag, premium grade(SH7-H5229) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Gly 197 (Accession # NP_000184).
    Predicted N-terminus: Cys 24
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    Sonic Hedgehog Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 20.4 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.2 EU per μg by the LAL method.
  • Sterility
    Negative
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Sonic Hedgehog SDS-PAGE

Human Sonic Hedgehog, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Application Data
 Sonic Hedgehog APPLICATION DATA

Human Sonic Hedgehog, His Tag, premium grade (Cat. No. SH7-H5229) and Human Noggin Protein, premium grade (Cat. No. NON-H5219) could efficiently induce the iPS cell into neuron progenitor cell differentiation, highly expressed PAX6, SOX1 and Nestin in immunofluorescence staining and FACS (Routinely tested).

 Sonic Hedgehog APPLICATION DATA

GMP Human FGF-8b Protein (Cat. No. GMP-FGBH16) and Human Sonic Hedgehog, His Tag, premium grade (Cat. No. SH7-H5229) could efficiently induce the neuron progenitor cell into dopaminergic neurons differentiation, highly expressed TH1 and MAP2 in immunofluorescence staining and FACS (Routinely tested).

  • Background
    Sonic hedgehog protein (SHH) is also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $195.00

Price(USD) : $1820.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:Phase 2 Clinical

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