Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
Biotinylated Human HLA-A*02:01&B2M&KRASG12V (KLVVVGAVGV) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12V peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human HLA-A*02:01&B2M&KRASG12V (KLVVVGAVGV) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human HLA-A*02:01&B2M&KRASG12V (KLVVVGAVGV) Complex Protein (Cat. No. HLV-H82E3) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
Immobilized Biotinylated Human HLA-A*02:01&B2M&KRASG12V (KLVVVGAVGV) Complex Protein (Cat. No. HLV-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Biotinylated Human HLA-A*02:01&B2M&KRASG12V (KLVVVGAVGV) Complex Protein (Cat. No. HLV-H82E3) at 1 μg/mL (100 μL/well) on (Cat. No. STN-N5116) streptavidin precoated (0.5 μg/well) plate can bind Anti-HLA-A2&KRAS G12V Antibody, Human IgG1 with a linear range of 0.2-1 ng/mL (QC tested).
Anti-HLA-A2/KRAS G12V Antibody captured on Protein A Chip can bind Biotinylated Human HLA-A*02:01&B2M&KRASG12V (KLVVVGAVGV) Complex Protein (Cat. No. HLV-H82E3) with an affinity constant of 37.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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