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Your Position: Home > Protein > Nectin-4 > NE4-H82F4

Biotinylated Human Nectin-4 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
  • Source
    Biotinylated Human Nectin-4 Protein, Fc,Avitag(NE4-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).
    Predicted N-terminus: Gly 32
  • Molecular Characterization
    Nectin-4 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 62.2 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Nectin-4 SDS-PAGE

Biotinylated Human Nectin-4 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Nectin-4 MALS images

The purity of Biotinylated Human Nectin-4 Protein, Fc,Avitag (Cat. No. NE4-H82F4) is more than 90% and the molecular weight of this protein is around 115-145 kDa verified by SEC-MALS.

 Nectin-4 ELISA

Immobilized Anti-Nectin-4 Antibody, Mouse IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-4 Protein, Fc,Avitag (Cat. No. NE4-H82F4) with a linear range of 0.2-8 ng/mL (QC tested).

 Nectin-4 ELISA

Immobilized Biotinylated Human Nectin-4 Protein, Fc,Avitag (Cat. No. NE4-H82F4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Nectin-4 Antibody, Mouse IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

  • Background
    Nectin-4(Nectin cell adhesion molecule 4) is a Nectin and nectin-like family of cell adhesion molecules. It is found in high levels in normal embryonic and fetal tissues, but decreases after adulthood and is limited in distribution in healthy tissues. Nectin-4 is overexpressed in a variety of tumor cells and promotes tumor cell growth and proliferation. Solid tumors with high expression of Nectin-4 include bladder cancer, pancreatic cancer, triple negative breast cancer, non-small cell lung cancer, stomach cancer, esophageal cancer, ovarian cancer and many other cancers. Studies have found that Nectin-4 promotes tumor cell proliferation, differentiation, migration and invasion by activating the PI3K/Akt pathway, and is believed to play an important role in the occurrence and metastasis of cancer. It is based on these research backgrounds that drug research targeting this target has emerged.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:10 Details
  • Latest Research Phase:Approved

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