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Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 66 kDa, 55 kDa, 41-43 kDa, 34 kDa, 27 kDa and 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).
Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) captured on CM5 Chip via Anti-B2M antibody can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with an affinity constant of 0.363 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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