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Your Position: Home > Protein > HLA-A*0301 & B2M & KRAS (VVVGAGGVGK) > HLS-H82E2

Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Monomer, MALS verified)

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-A*0301 & B2M & KRAS (VVVGAGGVGK)
  • Source
    Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein(HLS-H82E2) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGAGGVGK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGAGGVGK).
    Predicted N-terminus: Gly 25 & Ile 21
  • Molecular Characterization

    Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRAS peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 66 kDa, 55 kDa, 41-43 kDa, 34 kDa, 27 kDa and 11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-A*0301 & B2M & KRAS (VVVGAGGVGK) SDS-PAGE

Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
HLA-A*0301 & B2M & KRAS (VVVGAGGVGK) MALS images

The purity of Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 HLA-A*0301 & B2M & KRAS (VVVGAGGVGK) ELISA

Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

 HLA-A*0301 & B2M & KRAS (VVVGAGGVGK) ELISA

Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

Bioactivity-SPR
 HLA-A*0301 & B2M & KRAS (VVVGAGGVGK) SPR

Biotinylated Human HLA-A*03:01&B2M&KRAS (VVVGAGGVGK) Complex Protein (Cat. No. HLS-H82E2) captured on CM5 Chip via Anti-B2M antibody can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with an affinity constant of 0.363 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    The Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) oncogene plays a critical role in the initiation and maintenance of pancreatic tumors and its signaling network represents a major target for therapeutic intervention. The Biotinylated Human HLA-A*0301 KRAS (VVVGAGGVGK) complex protein is a complex of HLA-A*0301 of the MHC Class I, B2M, and VVVGAGGVGK peptide of the KRAS.
  • Clinical and Translational Updates

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