Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag
The protein has a calculated MW of 89.2 kDa. The protein migrates as 90-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human 4-1BB (71-254) Ligand, Fc,Avitag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human 4-1BB (71-254) Ligand, Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) is more than 85% and the molecular weight of this protein is around 180-210 kDa verified by SEC-MALS.
Immobilized Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) at 2 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB (71-254) Ligand, Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) with a linear range of 0.1-8 ng/mL (QC tested).
Immobilized Biotinylated Human 4-1BB (71-254) Ligand, Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) with a linear range of 0.2-16 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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