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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 89.2 kDa. The protein migrates as ＞180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human 4-1BB Ligand (71-254), Fc,Avitag, premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human 4-1BB Ligand (71-254), Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) is more than 90% and the molecular weight of this protein is around 180-220 kDa verified by SEC-MALS.
Immobilized Human 4-1BB Protein, Mouse IgG2a Fc Tag (Cat. No. 41B-H5256) at 2 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB (71-254) Ligand, Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) with a linear range of 0.2-6 ng/mL (QC tested).
Biotinylated Human 4-1BB Ligand (71-254), Fc,Avitag, premium grade (Cat. No. 41L-H82Fa) captured on Protein A Chip can bind Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) with an affinity constant of 342 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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