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Product Details
Synonyms
Apolipoprotein E, APOE, Apo-E
Source
Cynomolgus Apolipoprotein E Protein, His Tag (APE-C52H7) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P10517).
Predicted N-terminus: Lys 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.9 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus Apolipoprotein E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Cynomolgus Apolipoprotein E Protein, His Tag (Cat. No. APE-C52H7) is more than 85% and the molecular weight of this protein is around 165-185 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Cynomolgus TREM2, His Tag (Cat. No. TR2-C52H3) immobilized on CM5 Chip can bind Cynomolgus Apolipoprotein E Protein, His Tag (Cat. No. APE-C52H7) with an affinity constant of 7.78 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Cynomolgus Apolipoprotein E Protein, His Tag (Cat. No. APE-C52H7) immobilized on CM5 Chip can bind Cynomolgus TREM2, His Tag (Cat. No. TR2-C52H3) with an affinity constant of 4.54 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Apolipoprotein E
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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