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This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 47.9 kDa. The protein migrates as 45-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Tau-441 GSK-3beta-phosphorylated protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) at 1 μg/mL (100 μL/well) can bind Anti-Phospho-Tau (Thr181) Monoclonal Antibody, Mouse IgG with a linear range of 1-31 ng/mL (QC tested).
Immobilized Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) at 1 μg/mL (100 μL/well) can bind Anti-Tau Antibody with a linear range of 0.1-8 ng/mL (Routinely tested).
Mass spectrometry reveals 8 phosphorylation sites on E.coli expressed Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) as shown on the modified phosphorylation PTM spectrum. The protein sequence coverage was 40% (Routinely tested).
Western blot was performed by loading Human Tau-441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147): 400ng, and it was electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel. Human Tau 441 GSK-3beta-phosphorylated protein, His Tag (Cat. No. TAU-H5147) were transferred onto a pvdf membrane by Tanon Trans-Blot. Anti-Phospho-Tau (Thr181) Monoclonal Antibody is primary antibody (1 μg/mL) and (MFC) Peroxidase Affini Pure Goat Anti-Mouse IgG, Fcγ Fragment Specific (minXHu, Bov, HrSrProt (1:1500)) is second antibody detected by chemiluminescence with using the iBright 1500 (Chemiluminescent detection was performed using BeyoECL Plus).
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