Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein

Synonyms

HLA-A*0301 & B2M & KRASG12C (VVVGACGVGK)

Source

Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein (HLC-H52H9) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) & VVVGACGVGK peptide (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) & VVVGACGVGK).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Tetramer Protein is assembled by biotinylated monomer (HLC-H82E3) and streptavidin.

Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein is produced by co-expression of HLA and B2M loaded with KRASG12C peptide. Biotinylated Human HLA-A*03:01&B2M&KRASG12C (VVVGACGVGK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 35.9 kDa and 11.7 kDa. The protein migrates as 40-43 kDa, 13 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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