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Your Position: Home > Protein > Streptavidin > STN-NP117

Streptavidin Protein-PE, premium grade

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  • Synonym
    Streptavidin,SA
  • Source
    Streptavidin Protein-PE, premium grade(STN-NP117) is expressed from E. coli cells.
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization

    This protein carries no "tag".

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Endotoxin
    Less than 0.2 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M NaCl, 15 mg/ml HSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-Stability
 Streptavidin STABILITY

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

Bioactivity-FACS
 Streptavidin FACS

5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
  • Clinical and Translational Updates

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