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Product Details
Synonyms
Streptavidin, SA
Source
Streptavidin Protein-PE, premium grade (STN-NP117) is expressed from E. coli cells.
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Molecular Characterization
This protein carries no "tag".
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
1. Fluorescence based flow cytometric cell sorting.
2. Cell staining for flow cytometry analysis.
Endotoxin
Less than 0.5 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M NaCl, 15 mg/ml rHSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-Flow Cytometry
5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Protocol
Bioactivity-Stability
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291), Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-PE, premium grade (Cat. No. STN-NP117) and analyzed with FACS. PE signal was used to evaluate the binding activity.
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
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