Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (MALS verified)

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Cat. No. / Size
Price
Qty
H3L-M611-100ug
$415.00
H3L-M611-1mg (500ug X 2)
$2735.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    1C5

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Virus

  • Immunogen

    Recombinant Monkeypox virus (strain Zaire-96-I-16) H3L is expressed from human 293 cells

  • Specificity

    Specifically recognizes Monkeypox virus (strain Zaire-96-I-16) H3L.

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     H3L (MPXV) ELISA

    Immobilized Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     H3L (MPXV) SPR

    Monoclonal Anti-Monkeypox-H3L Antibody, Human IgG1 (1C5) (Cat. No. H3L-M611) captured on Protein A Chip can bind Monkeypox virus (strain Zaire-96-I-16) H3L, His Tag (Cat. No. H3L-M52H1) with an affinity constant of 0.194 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. H3L is similar to Vaccinia virus strain Copenhagen H3L heparin binding surface protein (Cop-H3L) surface membrane protein. There are research reports H3L induced transcriptional perturbations and injuries.

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