This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 43.7 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse TNF-alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Mouse TNF-alpha Protein, Fc Tag (Cat. No. TNA-M5257) is more than 95% and the molecular weight of this protein is around 255-275 kDa verified by SEC-MALS.
Immobilized Mouse TNF-alpha Protein, Fc Tag (Cat. No. TNA-M5257) at 5 μg/mL (100 μL/well) can bind Human TNFR1, His Tag (Cat. No. TN1-H5222) with a linear range of 1-16 ng/mL (QC tested).
Immobilized Human TNFR1, His Tag (Cat. No. TN1-H5222) at 5 μg/mL (100 μL/well) can bind Mouse TNF-alpha Protein, Fc Tag (Cat. No. TNA-M5257) with a linear range of 0.1-2 ng/mL (Routinely tested).
Immobilized Biotinylated Human TNFR2, His,Avitag (Cat. No. TN2-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse TNF-alpha Protein, Fc Tag (Cat. No. TNA-M5257) with a linear range of 0.1-1 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $300.00
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