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CHO/Human LIGHT Stable Cell Line Development Service

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  • Description
    The CHO/Human LIGHT Stable Cell Line was engineered to express full length human LIGHT (Gene ID: 8740). Surface expression of human LIGHT was confirmed by flow cytometry.
  • Application

    • Useful for cell-based LIGHT binding assay

    • Useful as LIGHT-expressing target cells in reporter gene assay

     LIGHT Assay Principles

  • Growth Properties
    Adherent
  • Selection Marker
    Puromycin (2 μg/mL)
  • Culture Medium
    F-12K + 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Mycoplasma Testing
    Negative
  • Sterility Testing
    Negative
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 LIGHT FACS

Expression analysis of human LIGHT on CHO/Human LIGHT Stable Cell Line by FACS.
CHO/Human LIGHT Stable Cell Line or negative control cell were stained with PE-labeled anti-Human LIGHT

Signaling Bioassay
 LIGHT SIGNALING

Response to human HVEM (FOLD).
This cell was incubated with Human HVEM (Luc) HEK293 Reporter Cell (Cat.No.CHEK-ATF105). The human LIGHT overexpressing on CHO cells can activate HVEM signaling with the max induction fold 227.59.

Passage Stability
 LIGHT PASSAGE

Passage stability analysis of receptor expression by FACS.
Flow cytometry surface staining of human LIGHT on CHO/Human LIGHT Stable Cell Line demonstrates consistent mean fluorescent intensity across passage 4-24

Please contact us if you are interested in related cell pool service.

  • Background
    Tumor necrosis factor ligand superfamily member 14(LIGHT) is a tumor necrosis factor (TNF) superfamily ligand that regulates T cell immune responses by signaling through the herpes virus entry mediator (HVEM) and the lymphotoxin beta receptor (LTbetaR). LIGHT has emerged as a potent initiator of T cell co-stimulation signals effecting CTL-mediated tumor rejection, allograft rejection and graft versus host disease. Constitutive expression of LIGHT leads to tissue destruction and autoimmune-like disease syndromes.
  • License Disclosure
    This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $3500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 2 Clinical

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