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Your Position: Home > Protein > Envelope protein E (JEV) > ENE-J52H5

Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified)

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  • Source
    Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified)(ENE-J52H5) is expressed from human 293 cells (HEK293). It contains AA Met 128 - Ala 700 (Accession # P27395-1 ).
  • Molecular Characterization
    Envelope protein E (JEV) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 64.6 kDa. The protein migrates as 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Envelope protein E (JEV) SDS-PAGE

Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Envelope protein E (JEV) MALS images

The purity of Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (Cat. No. ENE-J52H5) is more than 85% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Envelope protein E (JEV) ELISA

Immobilized Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified) (Cat. No. ENE-J52H5) at 1 μg/mL (100 μL/well) can bind JEV E-D3 antibody ,Mouse IgG with a linear range of 0.1-2 ng/mL (QC tested).

  • Background
    Japanese encephalitis B, a mosquito-borne flavivirus, or B encephalitis, is the most important cause of viral encephalitis in Asia in terms of frequency and severity. JEV envelope protein E is an important research target, binding to host cell surface receptors and mediating fusion between the virus and the cell membrane.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $450.00

Price(USD) : $2680.00

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