This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 39.2 kDa. The protein migrates as 48-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-15 Protein, Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human IL-15 Protein, Fc Tag, premium grade (Cat. No. IL5-H5257) is more than 95% and the molecular weight of this protein is around 80-100 kDa verified by SEC-MALS.
Human IL-15 Protein, Fc Tag, premium grade (Cat. No. IL5-H5257) stimulates proliferation of CTLL-2 cells. The typically EC50 for this effect is 2.483 ng/mL(QC tested).
Immobilized Human IL-15 Protein, Fc Tag, premium grade (Cat. No. IL5-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) with a linear range of 0.1-4 ng/mL (QC tested).
Immobilized Human IL-15 Protein, Fc Tag, premium grade (Cat. No. IL5-H5257) at 2 μg/mL (100 μL/well) can bind Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) with a linear range of 0.039-0.078 μg/mL (Routinely tested).
Biotinylated Human IL-15 R alpha, Fc,Avitag (Cat. No. ILA-H82F4) immobilized on CM5 Chip can bind Human IL-15 Protein, Fc Tag, premium grade (Cat. No. IL5-H5257) with an affinity constant of 27.7 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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