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Your Position: Home > All Other Proteins > CD58 > LF3-H5225

Human CD58 / LFA-3 Protein, His Tag

  • Synonym
    CD58,LFA3,Ag3
  • Source
    Human CD58, His Tag (LF3-H5225) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Arg 215 (Accession # AAH05930).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    Online(Phe 29  -  Arg 215) AAH05930

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD58, His Tag (Cat. No. LF3-H5225) SDS-PAGE gel

Human CD58, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD58, His TagHuman CD58, His Tag (Cat. No. LF3-H5225) ELISA bioactivity

Immobilized Human CD58, His Tag (Cat. No. LF3-H5225) at 10 μg/mL (100 μL/well) can bind Human CD2, Fc Tag (Cat. No. CD2-H5258) with a linear range of 5-78 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD2, Fc Tag (Cat. No. CD2-H5258) on Protein A Biosensor, can bind Human CD58, His Tag (Cat. No. LF3-H5225) with an affinity constant of 2.3 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58 /LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) [3] on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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