Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 39.3 kDa. The protein migrates as 52-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 50 mM Nacl, 1 mM EDTA, 2 mM DTT, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Galectin-9, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Human TIM-3 (22-200) Protein, Fc Tag (Cat. No. TM3-H5258) immobilized on CM5 Chip can bind Biotinylated Human Galectin-9, His,Avitag (Cat. No. LG9-H82H3) with an affinity constant of 7.66 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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