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This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 42.2 kDa. The protein migrates as 45-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, 0.5M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human APRIL Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 5 μg/mL (100 μL/well) can bind Human APRIL Protein, Fc Tag (Cat. No. APL-H5263) with a linear range of 0.06-2 ng/mL (QC tested).
Immobilized Human APRIL Protein, Fc Tag (Cat. No. APL-H5263) at 2 μg/mL (100 μL/well) can bind Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) with a linear range of 0.1-1 ng/mL (Routinely tested).
Immobilized Human APRIL Protein, Fc Tag (Cat. No. APL-H5263) at 2 μg/mL (100 μL/well) can bind Biotinylated Human TACI, Fc,Avitag (Cat. No. TAI-H82F6) with a linear range of 1-39 ng/mL (Routinely tested).
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