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Product Details
Synonyms
IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1
Source
Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (ILR-H525b) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 & Gly 22 - Thr 343 (Accession # P24394-1 & NP_001551.1).
Predicted N-terminus: Met 26 & Gly 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag is produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 50.2 kDa & 63.1 kDa. Subunit IL-4 R alpha is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-13 R alpha 1 is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) is more than 95% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) at 5 μg/mL (100 μL/well) can bind Human IL-4, His Tag (Cat. No. IL4-H52H9) with a linear range of 0.6-5 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-4 R alpha & IL-13 R alpha 1 Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525b) with a linear range of 0.6-20 ng/mL (Routinely tested).
Protocol
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