Human EBI3 Protein, His Tag

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Cat. No. / Size
Price
Qty
EB3-H51H3-100ug
$360.00
EB3-H51H3-1mg (200ug X 5)
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    IL-27B, IL27B, IL35B

  • Source

    Human EBI3 Protein, His Tag (EB3-H51H3) is expressed from E. coli cells. It contains AA Arg 21 - Lys 229 (Accession # Q14213).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    EBI3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.3 kDa. The protein migrates as 25-26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM NaAc, 150 mM NaCl, pH3.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    EBI3 SDS-PAGE

    Human EBI3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     EBI3 ELISA

    Immobilized Human IL-27 Protein, Fc Tag, premium grade (Cat. No. IL7-H5254) at 10 μg/mL (100 μL/well) can bind Human EBI3 Protein, His Tag (Cat. No. EB3-H51H3) with a linear range of 0.02-0.625 μg/mL (QC tested).

    Protocol

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Background

This gene was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells.

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