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Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (MALS verified)

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GHL-V5283-50ug
$465.00
In Stock - Delivery in 1-2 business days
GHL-V5283-500ug
$2,885.00
In Stock - Delivery in 2 weeks
GHL-V5283
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Product Details
Performance Data
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Glycoprotein H & Glycoprotein L/gH & gL (VZV)

Source

Varicella zoster virus (strain Oka vaccine) gH&gL Protein, Twin-Strep Tag&His Tag (GHL-V5283) is expressed from human 293 cells (HEK293). It contains AA Asn 18 - Arg 795 & Leu 23 - Gln 160 (Accession # Q775J3 & Q9J3N1).

Predicted N-terminus: Asn 18 & Leu 23

Molecular Characterization

This protein carries a twin strep tag at the C-terminus. The protein has a calculated MW of 90.2 kDa & 17 kDa. The protein migrates as 110-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 0.5 M Arginine, PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Acts as a functional inhibitor of gH and maintains gH in an inhibited form. Upon binding to host integrins, gL dissociates from gH leading to activation of the viral fusion glycoproteins gB and gH.