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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.5 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human DR3 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 10 μg/mL (100 μL/well) can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 1-78 ng/mL (QC tested).
Immobilized Human DR3, Fc Tag (Cat. No. DR3-H5253) at 0.6 μg/mL (100μL/well) can bind pre-mixed increasing concentrations of Anti-TL1A Neutralizing Antibody, Human IgG1 and 1μg/mL (50 μL/well) Biotinylated Human TL1A, His Avitag (Cat. No. TLA-H52Q1) with a half maximal inhibitory concentration (IC50) of 1.95 μg/mL (Routinely tested).
Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) captured on Protein A Chip can bind Human TL1A, His Tag (Cat. No. TLA-H5243) with an affinity constant of 7.58 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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