Human CD46 Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CD6-H5254-100ug
$390.00
CD6-H5254-1mg
$2595.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD46, AHUS2, MCP, MIC10, TLX, TRA2.10

  • Source

    Human CD46 Protein, Fc Tag (CD6-H5254) is expressed from human 293 cells (HEK293). It contains AA Cys 35 - Lys 159 (Accession # P15529-1).

    Predicted N-terminus: Cys 35

    Request for sequence
  • Molecular Characterization

    CD46 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 40.8 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD46 SDS-PAGE

    Human CD46 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CD46 SEC-MALS

    The purity of Human CD46 Protein, Fc Tag (Cat. No. CD6-H5254) is more than 95% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CD46 ELISA

    Immobilized Human CD46 Protein, Fc Tag (Cat. No. CD6-H5254) at 1 μg/mL (100 μL/well) can bind FG-3246 (FOR46), Human IgG1 kappa with a linear range of 0.1-8 ng/mL (QC tested).

    Protocol

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Background

Complement regulatory protein CD46 is also known as membrane Cofactor Protein (MCP), is a type I membrane protein and is a regulatory part of the complement system. CD46 is expressed by all cells except erythrocytes. MCP acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue, and also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. In T-cells by binding to CD46, A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2).

Recent Advances

 
Drug Development Progress
  • English Name:

    Membrane cofactor protein

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    5 Details

  • Highest Development Stage:

    Phase 2 Clinical

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