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Your Position: Home > Protein > IgG4 Fc > IG4-H5205

Human IgG4 Fc Protein, Tag Free (MALS & SPR verified)

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  • Synonym
    IGHG4
  • Source
    Human IgG4 Fc, Tag Free (IG4-H5205) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 327 (Accession # P01861).
    Predicted N-terminus: Glu 99
  • Molecular Characterization
    Online(Glu 99 - Lys 327) P01861

    This protein carries no "tag".

    The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) SDS-PAGE gel

Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IgG4 Fc, Tag Free (Cat. No. ) MALS images

The purity of Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) is more than 95% and the molecular weight of this protein is around 54-66 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (Cat. No. IG4-H5205) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (QC tested).

  • Background
    Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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