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Your Position: Home > Protein > CGRPR & RAMP1 > CG1-H52P6

Human CGRPR&RAMP1 Full Length Heterodimer Protein (VLP)

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  • Source
    Human CGRPR&RAMP1 Full Length Heterodimer Protein (VLP)(CG1-H52P6) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Asn 461 (CGRPR) & Cys 27 - Val 148 (RAMP1) (Accession # Q16602-1 (CGRPR) & O60894-1 (RAMP1)).
    Predicted N-terminus: Asp & Trp
  • Molecular Characterization

    The protein has a calculated MW of 53.6 kDa | 17.3 kDa | 56.1 kDa.

    Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

    The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

    Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-N5213) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 CGRPR & RAMP1 ELISA

Immobilized Human CGRPR&RAMP1 Full Length Heterodimer Protein (VLP) (Cat. No. CG1-H52P6) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CGRPR antibody, human IgG with a linear range of 0.5-8 ng/mL (QC tested).

Identity-DLS
 CGRPR & RAMP1 DLS

The mean peak Radius of VLP is 95-120 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

  • Background
    This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative R splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Clinical and Translational Updates

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