Human CD84 / SLAMF5 Protein, His Tag

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Cat. No. / Size
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CD4-H5229-100ug
$275.00
In Stock - Delivery in 2-3 business days
CD4-H5229-1mg (250ug X 4)
$1700.00
In Stock - Delivery in 2-3 business days

Product Details

  • Synonyms

    CD84, SLAMF5, LY9B, SLAMF5

  • Source

    Human CD84, His Tag (CD4-H5229) is expressed from human 293 cells (HEK293). It contains AA Lys 22 - Gly 225 (Accession # AAH20063).

    Predicted N-terminus: Lys 22

    Request for sequence
  • Molecular Characterization

    CD84 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 23.6 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CD84 SDS-PAGE

    Human CD84, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     CD84 ELISA

    Immobilized Human CD84, His Tag (Cat. No. CD4-H5229) at 1 μg/mL (100 μL/well) can bind various dilution ratio of CD84 Recombinant Rabbit Monoclonal Antibody, Rabbit IgG (QC tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background

Leukocyte differentiation antigen CD84 is also known as SLAM family member 5 (SLAMF5), which belongs to immunoglobulin (Ig) superfamily. CD84 / SLAMF5 contains one Ig-like C2-type (immunoglobulin-like) domain. CD84 plays a role as adhesion receptor functioning by homophilic interactions and by clustering. CD84 / SLAMF5 increases proliferative responses of activated T-cells and SH2D1A/SAP does not seen be required for this process. Homophilic interactions enhance interferon gamma/IFNG secretion in lymphocytes and induce platelet stimulation via a SH2D1A/SAP-dependent pathway.

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