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Product Details
Synonyms
HLA-G, HLA class I histocompatibility antigen, alpha chain G, HLAG, HLA-6.0
Source
Human HLA-G protein, His Tag (HLG-H51H3) is expressed from E. coli cells. It contains AA Gly 25 - Ala 206 (Accession # P17693-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23 kDa. The protein migrates as 23-26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, 0.5 M NaCl, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human HLA-G protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human HLA-G protein, His Tag (Cat. No. HLG-H51H3) at 5 μg/mL (100 μL/well) can bind Human KIR2DL4, Fc Tag (Cat. No. KI4-H5255) with a linear range of 1.6-50 ng/mL (QC tested).
Protocol
Immobilized Human HLA-G protein, His Tag (Cat. No. HLG-H51H3) at 5 μg/mL (100 μL/well) can bind Human LILRB2, Fc Tag (Cat. No. LI2-H5253) with a linear range of 0.3-2 ng/mL (Routinely tested).
Protocol
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