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| ID | Components | Size |
| AAVX01-C01 | AAV Capsid Capture Antibody | 120 μg |
| AAVX01-C02 | AAV Capsid Detection Antibody | 60 μg |
| AAVX01-C03 | HRP-Conjugated Antibody | 15 μg |
AAV Titration Detection Kit (Universal )was developed for the detection and quantitative determination of AAV capsid titration in AAV gene therapy product preparation processing.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 90 days from the date of opening.
Your experiment will include 5 simple steps:
a) Coat the plate with AAV Capsid Capture Antibody.
b) Add your molecule of interest to the tests.
c) Add AAV Capsid Detection Antibody.
d) Add HRP-Conjugated Antibody followed by TMB or other colorimetric HRP substrate.
e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis.
Immobilized AAV Capsid Capture Antibody (AAVX01-C01) at 1.0 μg/mL (100 μL/well) can bind AAV2 Capsid, and then add AAV Capsid Detection Antibody (AAVX01-C02) at 0.5 μg/mL (100 μL/well), final detection was performed using HRP-Conjugated Antibody (AAVX01-C03) with a linear range of 3.91E+07 - 2.50E+09 capsids/mL (QC tested).
Immobilized AAV Capsid Capture Antibody (AAVX01-C01) at 1.0 μg/mL (100 μL/well) can bind AAV Capsid, and then add AAV Capsid Detection Antibody (AAVX01-C02) at 0.5 μg/mL (100 μL/well), final detection was performed using HRP-Conjugated Antibody (AAVX01-C03).
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