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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 25.3 kDa. The protein migrates as 33-35 kDa and 37-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human LTBR Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Human LTBR Protein, His,Avitag (Cat. No. LTR-H52E3) is more than 90% and the molecular weight of this protein is around 25-38 kDa verified by SEC-MALS.
Immobilized Human LTBR Protein, His,Avitag (Cat. No. LTR-H52E3) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269) with a linear range of 0.06-0.5 ng/mL (QC tested).
Immobilized Human LIGHT Protein, Mouse IgG2a Fc Tag (Cat. No. LIT-H5256) at 5 μg/mL (100 μL/well) can bind Human LTBR Protein, His,Avitag (Cat. No. LTR-H52E3) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
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