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Product Details
Synonyms
LTBR, D12S370, TNFCR, TNFR3, TNFRSF3, TNFRIII
Source
Unconjugated Human LTBR Protein, His,Avitag (LTR-H52E3) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Met 227 (Accession # P36941-1).
Predicted N-terminus: Gln 31
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 25.3 kDa. The protein migrates as 33-35 kDa and 37-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Unconjugated Human LTBR Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Unconjugated Human LTBR Protein, His,Avitag (Cat. No. LTR-H52E3) is more than 95% and the molecular weight of this protein is around 25-38 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Unconjugated Human LTBR Protein, His,Avitag (Cat. No. LTR-H52E3) at 5 μg/mL (100 μL/well) can bind Human LIGHT Protein, Fc Tag (Cat. No. LIT-H5269) with a linear range of 0.06-0.5 ng/mL (QC tested).
Protocol
Immobilized Human LIGHT Protein, Mouse IgG2a Fc Tag (Cat. No. LIT-H5256) at 5 μg/mL (100 μL/well) can bind Unconjugated Human LTBR Protein, His,Avitag (Cat. No. LTR-H52E3) with a linear range of 0.039-0.625 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 3
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 1 Clinical
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