This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.8 kDa. The protein migrates as 44-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TIM-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human TIM-3, His Tag(Cat. No. TM3-H5229) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Anti-Human TIM-3 MAb (human IgG4) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human TIM-3, His Tag (Cat. No. TM3-H5229) with an affinity constant of 9.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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