Human Cathepsin D Protein, His Tag (Pro-form, MALS verified)

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Cat. No. / Size
Price
Qty
CAD-H52H3-50ug
$360.00
CAD-H52H3-1mg
$2165.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CTSD, Cathepsin D, CPSD, CLN10

  • Source

    Human Cathepsin D Protein, His Tag (CAD-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - Leu 412 (Accession # P07339-1).

    Predicted N-terminus: Leu 21

    Request for sequence
  • Molecular Characterization

    Cathepsin D Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 44.5 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 12.5 mM MES, 75 mM NaCl, pH6.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Cathepsin D SDS-PAGE

    Human Cathepsin D Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Cathepsin D SEC-MALS

    The purity of Human Cathepsin D Protein, His Tag (Cat. No. CAD-H52H3) is more than 95% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

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  • Bioactivity

    Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 . The specific activity is >350 pmol/min/µg (QC tested).

    Protocol

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Background

This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015]

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