Human GITR Ligand / TNFSF18 Protein, Fc Tag

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Cat. No. / Size
Price
Qty
GIL-H526a-50ug
$360.00
GIL-H526a-1mg
$2720.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    TNFSF18, AITRL, TL6, hGITRL, GITR Ligand

  • Source

    Human GITR Ligand, Fc Tag (GIL-H526a) is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1).

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  • Molecular Characterization

    GITR Ligand Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 41.5 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

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  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    GITR Ligand SDS-PAGE

    Human GITR Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     GITR Ligand ELISA

    Immobilized Human GITR, His Tag (Cat. No. GIR-H5228) at 2 μg/mL (100 μL/well) can bind Human GITR Ligand, Fc Tag (Cat. No. GIL-H526a) with a linear range of 2-80 ng/mL (QC tested).

    Protocol

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Background

Tumor necrosis factor ligand superfamily member 18 (TNFSF18) is also known as Glucocorticoid - induced TNF-related ligand (GITRL or GITR Ligand), Activation - inducible TNF - related ligand (AITRL), which belongs to the tumor necrosis factor family. TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. TNFSF18 / GITRL is up-regulated after stimulation by bacterial lipopolysaccharides (LPS). TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells. TNFSF18 also mediates activation of NF-kappa-B.

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Drug Development Progress
  • English Name:

    Tumor necrosis factor ligand superfamily member 18

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

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  • Highest Development Stage:

    Preclinical

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