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Product Details
Synonyms
HLA-A*1101 & B2M & WT1 (FSCRWPSCQK)
Source
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (HLW-HA2H4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) & FSCRWPSCQK peptide (Accession # Q5S3G3-1 (HLA-A*11:01) & P61769 (B2M) & FSCRWPSCQK).
Predicted N-terminus: Gly 25 & Ile 21
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein is assembled by biotinylated monomer and AF647-labeled streptavidin.
Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein is produced by co-expression of HLA and B2M loaded with WT-1 peptide. Biotinylated Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
Conjugate
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF647 using standard chemical labeling method. The residual AF647 is removed by molecular sieve treatment during purification process.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (Cat. No. HLW-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.12-2 ng/mL (QC tested).
Protocol
Immobilized Alexa Fluor 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer Protein (Cat. No. HLW-HA2H4) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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