Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein, Twin-Strep Tag&Tag Free

Synonyms

Integrin alpha 8 beta 1, ITGA8&ITGB1

Source

Human ITGA8&ITGB1 Heterodimer Protein, Twin-Strep Tag&Tag Free (IT1-H5283) is expressed from human 293 cells (HEK293). It contains AA Phe 39 - Leu 1012 & Gln 21 - Asp 728 (Accession # P53708-1 & P05556-1).

Predicted N-terminus: Phe 39 & Gln 21

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Human ITGA8&ITGB1 Heterodimer Protein, Twin-Strep Tag&Tag Free, produced by co-expression of ITGA8 and ITGB1, has a calculated MW of 116.4 kDa (ITGA8) and 83.7 kDa (ITGB1). Subunit ITGA8 is fused with Strep II-tag at the C-terminus and subunit ITGB1 contains no tag. The protein migrates as 150-180 kDa (ITGA8) and 95-110 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process