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Your Position: Home > Protein > Fc epsilon RI alpha > FCA-H82E3

Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag™ (MALS & BLI verified)

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  • Synonym
    FCER1A,FCE1A,FcERI
  • Source
    Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag(FCA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Val 26 - Gln 205 (Accession # P12319-1).
    Predicted N-terminus: Val 26
  • Molecular Characterization
    Fc epsilon RI alpha Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 24.6 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Fc epsilon RI alpha SDS-PAGE

Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Fc epsilon RI alpha MALS images

The purity of Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag (Cat. No. FCA-H82E3) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Fc epsilon RI alpha ELISA

Immobilized Human IgE Fc, His Tag (Cat. No. IGE-H52H9) at 1 μg/mL (100 μL/well) can bind Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag (Cat. No. FCA-H82E3) with a linear range of 0.02-0.6 μg/mL (QC tested).

Bioactivity-BLI
 Fc epsilon RI alpha BLI

Loaded Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag (Cat. No. FCA-H82E3) on SA Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 1.92 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
  • Clinical and Translational Updates

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  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Approved

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