Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET)

• Simple and fast operation - No complicated washing steps, significantly reducing time.
• High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
• Accurate and reliable results - High sensitivity with minimal matrix effects.
• High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
• Fast completion - Results in just 1 hour.

The Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screening for inhibitors of human Fc epsilon RI alpha binding to human IgE Fc within 0.5-1 hours. It can also be used as a universal detection tool to identify the ability of human Fc epsilon RI alpha to bind to IgE Fc.

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This Human Fc epsilon RI alpha / IgE Fc Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). Use a mixture of biotinylated human Fc epsilon RI alpha protein and FA labeled streptavidin as the acceptor and human IgE Fc protein Europium-chelate as the donor.

— In the absence of inhibitors for human Fc epsilon RI alpha protein binding to human IgE Fc protein, the donor and acceptor are in close proximity due to the binding of human Fc epsilon RI alpha protein and human IgE Fc protein. Upon excitation with a specific light source, the donor emits a 620 nm signal, which is absorbed by the acceptor, resulting in a 665 nm emission.

— In the presence of inhibitors that block the binding of human Fc epsilon RI alpha protein to human IgE Fc protein, the donor-acceptor interaction is disrupted, preventing FRET from occurring.