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Product Details
Synonyms
betaKlotho, beta-klotho, BKL, KLB, klotho beta like, klotho beta-like protein
Source
Human Klotho beta Protein, His Tag (KLB-H52H4) is expressed from human 293 cells (HEK293). It contains AA Met 30 - Leu 997 (Accession # Q86Z14-1).
Predicted N-terminus: Met 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 112.9 kDa. The protein migrates as 120-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Klotho beta Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human Klotho beta Protein, His Tag (Cat. No. KLB-H52H4) at 10 μg/mL (100 μL/well) can bind Human FGF19, Fc Tag (Cat. No. FG9-H5253) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Immobilized Human Klotho beta Protein, His Tag (Cat. No. KLB-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF-21 Protein, His,Avitag (Cat. No. FG1-H82Q3) with a linear range of 0.02-0.156 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Klotho beta Protein, His Tag (Cat. No. KLB-H52H4) immobilized on CM5 Chip can bind Human FGF-21, His Tag (Cat. No. FG1-H5243) with an affinity constant of 67.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Klotho beta
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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