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Your Position: Home > All Other Proteins > Osteoprotegerin > Human Osteoprotegerin / TNFRSF11B Protein, Fc Tag

Human Osteoprotegerin / TNFRSF11B Protein, Fc Tag

  • Synonym
    TNFRSF11B,OCIF,OPG,Osteoprotegerin
  • Source
    Human Osteoprotegerin, Fc Tag (TNB-H5259) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Leu 401 (Accession # NP_002537).
    Predicted N-terminus: Glu 22
  • Molecular Characterization
    Online(Glu 22 -  Leu 401) NP_002537

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 70.2 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) SDS-PAGE gel

Human Osteoprotegerin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Bioactivity-ELISA
Human Osteoprotegerin, Fc TagHuman Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) ELISA bioactivity

Immobilized Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 4-16 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human TRAIL, His Tag on HIS1K Biosensor, can bind Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) with an affinity constant of 1.65 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) on AHC Biosensor, can bind Human TRAIL, His Tag with an affinity constant of 2.32 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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