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Product Details
Synonyms
HLA-DRA1*0101 & HLA-DRB1*1101 & Influenza HA (PKYVKQNTLKLAT)
Source
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein (HLA-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*11:01) & PKYVKQNTLKLAT peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388099.1 (HLA-DRB1*11:01) & PKYVKQNTLKLAT).
Predicted N-terminus: Ile 26 & Gly 30
Request for sequenceMolecular Characterization
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with Influenza HA peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 29.2 kDa and 29.2 kDa. The protein migrates as 38-42 kDa and 35-36 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*11:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein (Cat. No. HLA-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).
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