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Product Details
Synonyms
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
Source
Human Transferrin R, His Tag (CD1-H5243) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # P02786-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 77.0 kDa. The protein migrates as 80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Mass Spectrometry
Sequence Coverage
Human Transferrin R, His Tag(CD1-H5243) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage.
N-terminal Sequence Analysis
(Tag)-Cys-Lys-Gly-Val-Glu-Pro-Lys-Thr-Glu-Cys-Glu-Arg-Gly-Val-Glu
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human Transferrin R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Transferrin R, His Tag (Cat. No. CD1-H5243) is more than 95% and the molecular weight of this protein is around 145-180 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Transferrin R, His Tag (Cat. No. CD1-H5243) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin Protein, His,Avitag (Cat. No. TRN-H82E3) with a linear range of 0.04-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Captured Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-MY2101) on CM5 chip via anti-mouse antibodies surface can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.821 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Brain-penetrant, anti-amyloid, bispecific antibody immobilized on CM5 Chip can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 94.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Trontinemab captured on Protein A Chip can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 33.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human Transferrin Protein, His,Avitag (Cat. No. TRN-H82E3) on SA Biosensor, can bind Human Transferrin R, His Tag (Cat. No. CD1-H5243) with an affinity constant of 0.143 nM as determined in BLI assay (ForteBio Octet RED96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
ZZ2022-10-11
LikesWe needed this product for our antibody research. ACRO managed to get it to us much faster than we expected, which made our research work very accurate. cd71 is a potential new target in human leukemia and lymphoma cases. cd71 /TFRC / TfR has been shown to interact with GABARAP and HFE. I am very pleased with the product and the quick technical support.- C***U2022-10-06 LikesThis product was needed for our antibody research. ACRO managed to deliver this much quicker than we expected which made the conduct of our research extremely accurate. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE. We really satisfied this product, and we absolutely order more products for our further projects.
- C***n2022-03-29 LikesI have used Transferrin R from Acro Biosystems as the ligand in developing SPR analyses where protein activity is vital and not always verified with certain vendors. It is additionally helpful to have reliable molecular weight, which I have found most vendors are not nearly as accurate with. Products are received in a timely manner and have good stability if stored appropriately. Very satisfied with the product and the quick technical support available!
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
Transferrin receptor protein 1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Approved
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