Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Kits > dsRNA > RES-A092

Double-Stranded RNA (dsRNA) ELISA Kit

For research use only.

Order Now

FAB
  1. High Sensitivity - Detects dsRNA at as low as 8.988 pg/mL, crucial for trace dsRNA detection in mRNA vaccine QC.
  2. High Specificity - Specifically identifies dsRNA, not cross - reacting with ssRNA or dsDNA for accurate detection.
  3. Broad Applicability - Recognizes >40bp dsRNA, including various modified types, suitable for diverse scenarios.
  4. Flexible usage - Customers can purchase 5-OMe-UTP dsRNA, N1-Me-pUTP dsRNA, and pUTP dsRNA standard according to their own needs.
  5. Good Precision and Accuracy - High intra-assay as well as inter-assay precision, and accurate recovery for high, middle, low-concentration samples to ensure reliable results.
    Product Specifications
    Assay TypeSandwich-ELISA
    AnalytedsRNA
    Format96T(8×12 strips)
    Reactivity/
    Regulatory StatusRUO
    Sensitivity8.988 pg/mL
    Standard Curve Range25 pg/mL-800 pg/mL
    Assay Time2 hr 20 min
    Suitable Sample TypeFor the detection and quantitative determination of dsRNA in samples
    Sample volume100 uL
  • Product Overview
    In mRNA vaccine development, dsRNA is a significant concern. During the in vitro transcription (IVT) of mRNA, dsRNA is frequently generated as a by - product. This occurs due to various factors like sequence structure, the nature of the RNA polymerase used, and the composition of the reaction system. DsRNA is a pathogen - associated molecular pattern (PAMP). It can be recognized by pattern - recognition receptors in the cytoplasm, which then initiate immune responses. This activation can lead to the inhibition of mRNA vaccine translation and its degradation. As a result, it has a direct impact on the safety and effectiveness of mRNA vaccines. For instance, it may trigger inflammation in vaccine recipients. Therefore, precisely detecting and controlling the amount of dsRNA in mRNA vaccines is crucial for guaranteeing vaccine quality and safety.
  • Application

    1.mRNA Vaccine Development

    2.Quality Control of IVT Products

    3.Research on Viral Infections

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Materials Provided
    IDComponentsSize
    RES092-C01Pre-coated Anti-dsRNA Monoclonal Antibody (K1) Microplate1 plate(8×12 strips)
    RES092-C02Unmodified dsRNA (400bp) Standard10 μg×2
    RES092-C03HRP-Anti-dsRNA Monoclonal Antibody (J2)10 μg×2
    RES092-C0410×Washing Buffer50 mL
    RES092-C05Sample Dilution Buffer50 mL
    RES092-C06HRP-Antibody (J2) Dilution Buffer25 mL
    RES092-C07Substrate Solution12 mL
    RES092-C08Stop Solution7 mL
Typical Data Please refer to DS document for the assay protocol.
 dsRNA TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

Validation
Intra-Assay Statistics

Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.

 dsRNA INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

 dsRNA INTER-ASSAY STATISTICS
Recovery

Three samples with different concentrations were tested to calculate the recovery rate.

 dsRNA RECOVERY
Specificity

The kit has been tested to specifically identify dsRNA, and do not identify ssRNA , dsDNA.

 dsRNA SPECIFICITY
  • Clinical and Translational Updates

Comments (0)


ETA of in-stock products: 2 business days

Price(USD) : $600.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message